Standards > reporting guideline > bsg-s000171


ready Minimum Information Specification For In Situ Hybridization and Immunohistochemistry Experiments

Abbreviation:MISFISHIE


General Information
MISFISHIE is the Minimum Information Specification For In Situ Hybridization and Immunohistochemistry Experiments. This specification details the minimum information that should be provided when publishing, making public, or exchanging results from visual interpretation-based tissue gene expression localization experiments such as in situ hybridization, immunohistochemistry, reporter construct genetic experiments (GFP/green fluorescent protein, β-galactosidase), etc. Compliance to this standard is expected to provide researchers at other labs enough information to reproduce the experiment and/or to fully evaluate the data upon which results are based.

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Record updated: Oct. 17, 2016, 2:04 p.m. by Madekale.



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Implementing Databases (3)
Expression Database in 4D
This database provides a platform to query and compare gene expression data during the development of the major model animals (zebrafish, drosophila, medaka, mouse). The high resolution expression data was acquired through whole mount in situ hybridsation-, antibody- or transgenic experiments.

Ascidian Network for In Situ Expression and Embryological Data
Aniseed is a database designed to offer a representation of ascidian embryonic development at the level of the genome (cis-regulatory sequences, spatial gene expression, protein annotation), of the cell (cell shapes, fate, lineage) or of the whole embryo (anatomy, morphogenesis).

e-Mouse Atlas
The e-Mouse Atlas (EMA) is a detailed model of the developing mouse. Its purpose is not only to provide information about the shape, gross anatomy and detailed histological structure of the mouse, but also to provide a framework into which information about gene function can be mapped. The spatial, temporal and anatomical integration achieved by mapping diverse data to the organism itself rather than simply to database tables, allows powerful computational analyses. The website presents 3D volumetric models of the embryo linked, in most cases, to comprehensive and detailed images of histological structure. The embryo models and anatomy in EMA are organized by standard developmental stage (TS. after Theiler, 1989) and age of the embryo in days post coitum (dpc.). Gene-expression and other image data is also available.

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Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE).

Deutsch EW,Ball CA,Berman JJ,Bova GS,Brazma A,Bumgarner RE,Campbell D,Causton HC,Christiansen JH,Daian F,Dauga D,Davidson DR,Gimenez G,Goo YA,Grimmond S,Henrich T,Herrmann BG,Johnson MH,Korb M,Mills JC,Oudes AJ,Parkinson HE,Pascal LE,Pollet N,Quackenbush J,Ramialison M,Ringwald M,Salgado D,Sansone SA,Sherlock G,Stoeckert CJ Jr,Swedlow J,Taylor RC,Walashek L,Warford A,Wilkinson DG,Zhou Y,Zon LI,Liu AY,True LD
Nat Biotechnol 2008

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Development of the Minimum Information Specification for In Situ Hybridization and Immunohistochemistry Experiments (MISFISHIE).

Deutsch EW,Ball CA,Bova GS,Brazma A,Bumgarner RE,Campbell D,Causton HC,Christiansen J,Davidson D,Eichner LJ,Goo YA,Grimmond S,Henrich T,Johnson MH,Korb M,Mills JC,Oudes A,Parkinson HE,Pascal LE,Quackenbush J,Ramialison M,Ringwald M,Sansone SA,Sherlock G,Stoeckert CJ Jr,Swedlow J,Taylor RC,Walashek L,Zhou Y,Liu AY,True LD
OMICS 2006

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